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Updated: Sep 15, 2025

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在空间环境中对单个单元格进行高分辨率映射.

Jincan Ke1,2, Jian Xu3,4, Jia Liu3

  • 1Center for Cell Lineage Technology and Engineering, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Nature communications
|July 15, 2025
PubMed
概括

具有位置特征的细胞映射 (CMAP) 通过整合单细胞和空间数据,精确地映射组织中的细胞. 这种方法克服了基因恢复和分辨率的局限性,用于详细的空间转录组学分析.

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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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