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在海的乳头表达调节网络:全转录组和DNA甲基化数据集

Wenhong Mai¹, Jingxian Sun^1,2, Bingyu Ning¹

¹Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning, 116023, P. R. China.

Scientific data

|July 15, 2025

概括

这项研究揭示了基因表达和表观遗传调节网络控制海黄瓜乳头大小. 这些发现为了解Apostichopus japonicus的乳头发育和选择性繁殖提供了基础.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

斑点大小是海 (Apostichopus japonicus) 的一个关键特征,影响了它们的商业价值.
了解调节乳头大小的分子机制对于水产养殖和选择性育种至关重要.

研究的目的:

为了阐明Apostichopus japonicus的乳头大小的表达调节网络.
为了确定关键的基因和表观遗传修饰涉及到乳头发育.

主要方法:

全转录组测序 (RNA测序) 用于分析基因表达.
全基因组双硫酸盐测序以生成DNA甲基组数据.
识别竞争的内源RNA (ceRNA) 网络和差异甲基化区域 (DMR).

主要成果:

产生了大规模的转录基 (16.35G基) 和甲基 (28.92G基) 数据集.
确定了3,188个ceRNA网络,包括长非编码RNA (lncRNA) /microRNA (miRNA) /mRNA和循环RNA (circRNA) /miRNA /mRNA相互作用.
在内子,促进子和外子中检测到许多差异甲基化区域 (DMR),表明表观遗传调节乳头大小.

结论:

该研究建立了一个理论基础,特别是从表观遗传的角度来看,了解海黄瓜的乳头尺寸调节.
生成的数据集作为一种有价值的资源,用于生物标志物发现,用于选择性繁殖计划,重点是乳头外观.

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