从小鼠纤维细胞诱导癌症干细胞
Snehal Shabrish1,2, Leimarembi Devi Naorem2,3,4, Shubham Mohanty1,2
1Translational Research Laboratory Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Scientific reports
|July 15, 2025
概括
来自癌症患者的无细胞色素颗粒 (cfChPs) 将健康细胞转化为癌症干细胞. 这些转变的细胞显示干细胞标志物并形成瘤,这表明cfChPs可以驱动转移.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 诱导癌症干细胞形成的自然生物制剂是未知的.
- 来自癌症患者血液的无细胞色素颗粒 (cfChPs) 可以导致健康细胞的DNA损伤和炎症.
研究的目的:
- 调查cfChPs是否可以诱导正常体细胞成为癌症干细胞.
- 描述由cfChPs转换的细胞的特性.
主要方法:
- NIH3T3小鼠纤维细胞用来自癌症患者血清的cfChPs进行治疗.
- 使用流式细胞计和球形形成试验来评估茎状标志物和属性.
- 转录组分析发现了高调的基因和通路.
- 在SCID小鼠中进行了体内瘤形成测试.
主要成果:
- 经cfChP处理的细胞 (克隆D5) 显示干细胞因子 (OCT4,SOX2,KLF4) 和标记物 (CD34,CD44,CD133) 的表达增加.
- 这些细胞形成球形,表明干细胞的行为.
- 转录组分析显示,干性相关基因 (Klf4) 和与癌症相关的途径 (ERBB信号,P53,ATM,亡) 的上调.
- 在小鼠中形成的瘤表达了内皮标记物alphafetoprotein,证实了干细胞的特性.
结论:
- cfChPs可以将敏感的体细胞转化为癌症干细胞.
- 这些发现表明了癌症干细胞生成的新机制以及在转移中的潜在作用.
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