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单细胞免疫学揭示了关于结脊髓炎患者发病和不同结果的免疫学特征

Dianshan Ke^1,2, Rongsheng Zhang¹, Hanhao Dai¹

¹Department of Orthopedics, Shengli Clinical Medical College of Fujian Medical University, Fuzhou University Affiliated Provincial Hospital, Fujian Provincial Hospital, Fuzhou , Fujian, 350003, China.

Clinical rheumatology

|July 15, 2025

概括

结节性脊柱炎 (AS) 涉及显著的T/B细胞克隆扩张,特别是B细胞驱动AS病变. 缓解中的V(D) J基因多样性减少表明,免疫反应不足有助于疾病的持续性.

科学领域:

免疫学免疫学免疫学
遗传学遗传学是一个
类风湿病学类风湿病学

背景情况:

结性脊柱炎 (AS) 背后的免疫机制仍然不完全理解.
识别特定的免疫细胞反应对于理解AS病原和进展至关重要.

研究的目的:

阐明与AS发生和发展相关的免疫学特征.
通过使用先进的测序技术,探索驱动AS发病和不同疾病结果的免疫模式.

主要方法:

从不同阶段的健康捐赠者和AS患者的外周血液单核细胞 (PBMC) 的单细胞RNA测序.
分析T细胞受体 (TCR) 和B细胞受体 (BCR) 谱系,包括V(D) J基因重组和CDR3序列分析.

主要成果:

在效应细胞和AS病变中观察到明显的T/B细胞克隆扩张,其中B细胞扩张是突出的.
在缓解期的AS患者中,T/B细胞克隆扩张减少和V(D) J基因重排和CDR3序列的多样性降低.
特定的T细胞受体 (TRAV,TRBV,TRAJ,TRBJ) 和B细胞受体 (IGHV,IGHJ,IGLV,IGLJ) 基因使用与不同的AS阶段有关,包括发病,恶化和缓解.

结论:

B细胞的克隆扩张是驱动AS病变的关键因素,BCR-V(D) J重组的特异性起着至关重要的作用.
降低AS缓解中的V(D) J使用多样性与不充分的克隆扩张有关,这表明治疗策略的潜在目标.
这些发现提供了关于AS动态免疫反应的见解,并强调了免疫谱系多样性的重要性,以实现和维持缓解.

关键词:

结性脊柱炎是一种疾病.在BCR中,BCR是指BCR.在 Onset 设置上.结果结果的结果.在TCR中,可以使用TCR.V (((D) J) J) 的时间.

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