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Updated: Sep 15, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

整体转录组测序以发现与精神分裂症病原发生有关的CircRNA表达模式

Jianxiong Long¹, Bing Shen¹, Fangping Liao²

¹Epidemiology and Biostatistics, School of Public Health, Guangxi Medical University, Nanning, Guangxi, China.

European journal of medical research

|July 15, 2025

概括

精神分裂症 (SCZ) 缺乏客观的诊断标记. 这项研究确定了三个上调循环RNA (circRNAs) hsa_circ_0006157,hsa_circ_0071422和hsa_circ_0071095 作为SCZ的潜在诊断指标.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
精神病学是一个精神病学.

背景情况:

精神分裂症 (SCZ) 的发病过程复杂,缺乏客观的诊断指标.
全转录组分析为早期诊断标记物发现提供了潜在的机会.
在SCZ中循环RNAs (circRNAs) 的作用在很大程度上仍未被探索.

研究的目的:

在SCZ患者的外周血液中研究差异表达的circRNAs.
为了确定SCZ.潜在的基于circRNA的诊断标记物.
探索涉及SCZ中circRNAs的调节网络.

主要方法:

来自5名SCZ患者和5名对照患者的周围血液的全转录组分析.
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
预测竞争的内源RNA (ceRNA) 网络,蛋白-蛋白相互作用和circRNA-RNA结合蛋白 (RBP) 相互作用.
定量实时PCR (qRT-PCR) 用于循环RNA表达的验证.

主要成果:

分析了38,699个circRNA,确定了589个差异表达的circRNA (DEcircRNA).
DEcircRNAs的宿主基因与TNF和Wnt信号通路有关.
构建了一个ceRNA网络;在SCZ患者中确定了三个显著上调的DEcircRNA (hsa_circ_0006157,hsa_circ_0071422,hsa_circ_0071095).
确定了27个RBP与DEcircRNAs结合,包括显著表达的DDX54和RBM15B.

结论:

上调的circRNAs hsa_circ_0006157,hsa_circ_0071422和hsa_circ_0071095显示出作为SCZ潜在的诊断标记物的希望.
需要进一步的功能研究来确认这些circRNAs在SCZ病变发生中的确切作用.

关键词:

案例 - 控制研究.在CeRNA网络中,循环RNA 是一个循环RNA.在 RBP 网络中,您可以使用 RBP.精神分裂症是一种精神分裂症.

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