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Hsa_circ_0001859通过miRNA-101-3p/MMP1轴促进NSCLC的进展

Jianxin Tan¹, Zhenyu Fan², Rongguo Lu¹

¹Lung Transplant Center, Department of Thoracic Surgery, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi, Jiangsu, China.

Frontiers in oncology

|July 16, 2025

概括

循环RNAcirc_0001859促进非小细胞肺癌 (NSCLC) 的生长和转移,通过海绵化miR-101-3p和上调MMP1. 这项研究揭示了circ_0001859作为NSCLC的潜在治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

非小细胞肺癌 (NSCLC) 在中国是一个重大的健康负担,许多患者在晚期被诊断出来.
在NSCLC病变发生过程中,外形圆形RNAcirc_0001859的特定作用仍然在很大程度上未被描述.

研究的目的:

研究circ_0001859在非小细胞肺癌中的功能和调节机制.
探索circ_0001859作为NSCLC的预后指标和治疗点的潜力.

主要方法:

定量实时聚合酶连锁反应 (qRT-PCR) 和西部分离被用于评估基因和蛋白质表达.
进行了细胞增殖,迁移和入侵试验 (CCK-8,殖民地形成,transwell).
路西法酶报告员,RNA免疫沉降 (RIP) 测试,以及体内异种移植模型被用来阐明分子机制.

主要成果:

Circ_0001859在NSCLC组织和细胞中显著过度表达.
沉默circ_0001859抑制了NSCLC细胞的增殖,迁移和入侵.
Circ_0001859作为miR-101-3p的分子海绵,调节MMP1表达并促进NSCLC的进展.

结论:

Circ_0001859通过miR-101-3p/MMP1通路促进NSCLC的生长和转移.
这项研究提供了第一次详细调查circ_0001859在NSCLC中的作用和机制.
Circ_0001859代表了一个有前途的预后生物标志物和NSCLC的治疗标.

关键词:

在MMP1中,MMP1是MMP1.环_000185959 在线观看在线观看在 miR-101-3p 中.非小细胞肺癌的肺癌.这是一个渐进的过程.

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