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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

272
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
272
CRISPR01:59

CRISPR

53.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.0K

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相关实验视频

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR RiPCA用于调查eIF4E-m7GpppX封顶的mRNA相互作用

Gabriela Vega-Hernández1, Jesse Duque2, Brandon J C Klein3

  • 1Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan 48109, United States.

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概括

研究人员开发了CRISPR RiPCA,这是研究活细胞中的RNA-蛋白相互作用的新平台. 该工具测量了向真核转化启动因子4E (eIF4E) RNA结合蛋白的癌症药物抑制剂的活性.

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科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 遗传学 是一个遗传学.

背景情况:

  • 转录后的mRNA修饰是由读者RNA结合蛋白 (RBPs) 调节的.
  • RBPs的失调与癌症,神经退行和病毒感染等疾病有关.
  • 向RBP提供了一种治疗策略,用于恢复正常的RNA处理和功能.

研究的目的:

  • 开发一种用于活细胞RNA-蛋白相互作用 (RPI) 分析的新平台.
  • 将CRISPR技术与RNA相互作用与蛋白介导补充试验 (RiPCA) 结合起来.
  • 创建一个工具来评估潜在治疗剂的活性.

主要方法:

  • 建立了一个名为CRISPR RiPCA的活细胞RPI测试平台.
  • 使用了与RiPCA试验集成的CRISPR技术.
  • 使用真核转化启动因子4E (eIF4E) 与m7G覆盖的RNA基质之间的相互作用建模了系统.

主要成果:

  • 证明了CRISPR RiPCA在测量目标活动方面的潜力.
  • 成功地应用了eIF4E CRISPR RiPCA来研究一个特定的RBP相互作用.
  • 展示了该技术对癌症药物发现的相关性.

结论:

  • 克里斯普尔RiPCA是一种强大的新平台,用于研究活细胞中的RPI.
  • 这项技术可以评估针对RBP的治疗干预措施.
  • 开发的平台对癌症药物发现和其他疾病领域具有重大前景.