在超的应力过程中,ER连接和活性传输控制了凝结物扩散
Bisal Halder1,2, Guoming Gao1,3, Armin Ahnoud1
1Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, USA.
bioRxiv : the preprint server for biology
|July 16, 2025
概括
超的冲击会导致细胞的压缩和生物分子凝结物的形成. 这项研究揭示了这些凝结物仍然是动态的和可访问的,由ER附着和活跃运输组织,挑战静态细胞底模型.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 分子生物学分子生物学
背景情况:
- 超透性冲击会诱导细胞体积的压缩,导致细胞质和核质中的超透性相分离 (HOPS) 凝聚物形成.
- 在超的压力下,这些生物分子凝聚物的动力学和调节尚未得到充分理解.
研究的目的:
- 系统地描述在超大气压缩细胞中HOPS凝结物的动态.
- 阐明控制凝结物运动和空间组织的机制.
主要方法:
- 活细胞单颗粒追踪 (SPT) 跨越不同的时间尺度.
- 测量空间可访问性和器官标签.
- 使用遗传编码的多重分子纳米粒子 (GEMs) 重建细胞可访问性地图.
主要成果:
- HOPS凝结物主要表现出亚扩散,其中一小部分表现出超扩散.
- 亚扩散源于内分泌网膜 (ER) 附着;超扩散与微管体依赖的活性运输有关.
- 压缩的细胞细胞质仍然是高度可访问的,缺乏显著的局部角质.
结论:
- 超体压缩细胞中的细胞醇是动态的和可访问的,与之前的假设相反.
- 凝聚物通过对接到膜结构和间歇性远程运输来空间组织.
- 这些发现为通过细胞结构和过程对凝聚物的时空组织提供了一个模型.
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