关于在CRC瘤微环境中ECM原-GAG的空间表征的协议
Libby A Boykin1, Sneha Jayashankar1, Khidr Kishan K Budhwani1
1CerFlux, Birmingham, AL, USA.
bioRxiv : the preprint server for biology
|July 16, 2025
概括
这项研究介绍了一种简单,强大的协议,用于在结直肠癌 (CRC) 瘤微环境中同时可视化原蛋白和糖氨基甘氨酸. 这种方法有助于通过绘制细胞外矩阵组织的地图来理解CRC进展和治疗耐药性.
科学领域:
- 生物医学工程 生物医学工程
- 癌症研究 癌症研究
- 组织病理学 组织病理学
背景情况:
- 细胞外基质 (ECM) 在结直肠癌 (CRC) 进展和治疗耐药性方面至关重要.
- 现有的ECM表征方法通常是复杂的或单独分析组件.
- 了解瘤微环境 (TME) 中的ECM组织对于治疗开发至关重要.
研究的目的:
- 在CRC TME中提出一个简单,可靠的协议,用于CRC TME中的原和糖氨基甘氨酸 (GAG) 组织的空间表征.
- 为了实现同时可视化,评估和量化原和GAG分布在甲固定,嵌组织.
- 为ECM分析提供标准化的方法,可以作为瘤模型的基准.
主要方法:
- 结合Alcian蓝色和Picrosirius红色染色与标准化组织处理和成像.
- 开发了一种优化的双染色方法,用于明显分离原蛋白和GAG的光谱.
- 经过验证的成像参数和试剂制剂,以获得可重复的结果.
主要成果:
- 实现了对原和GAG分布模式的同时可视化和量化.
- 鲜明的蓝红色调允许简单的光谱分离.
- 通过使用标准组织学设备,在多个CRC患者标本中证明了出色的可复制性和一致的染色强度.
结论:
- 开发的协议为CRC TME的空间ECM表征提供了一种实用和可重复的方法.
- 这种方法促进了TME研究,支持了形态学研究,并使CRC亚型之间的比较分析成为可能.
- 该方法可适应其他固体瘤,并可与数字病理学工作流程集成,以进行增强分析.
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