来自Bacteroides fragilis的外膜囊泡含有编码和非编码的小RNA物种,这些物种调节肠道上皮细胞中的炎症信号
Aadil Sheikh1, Colin Scano1, Julia Xu1
1Department of Biology, Baylor University, Waco, USA.
bioRxiv : the preprint server for biology
|July 16, 2025
概括
来自致病菌Bacteroides fragilis的外膜囊泡 (OMV) 含有抑制免疫反应的RNA,而它们的去除会增强炎症. 这揭示了微生物衍生RNA在宿主免疫力中的双重作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 微生物组的改变会影响宿主生理和免疫反应.
- 外膜囊泡 (OMVs) 中微生物衍生的RNAs对宿主免疫的作用尚不清楚.
- 细菌菌 (Bacteroides fragilis),既有肠毒性 (ETBF),也有非毒性 (NTBF),是关键的肠道病原体/病原体.
研究的目的:
- 调查OMVs及其相关小RNAs (sRNAs) 从ETBF和NTBF调节肠上皮细胞免疫反应中的作用.
- 描述ETBF和NTBFOMVs的sRNA配置文件及其差异表达.
- 确定OMVs对模式识别受体激活和细胞因子产生的影响.
主要方法:
- RNA测序 (RNA-seq),qRT-PCR和北方涂抹,以描述和量化OMV中的sRNA.
- 报告器细胞测试以评估OMVs的托尔类受体 (TLR) 激活.
- 用OMV治疗肠上皮细胞系 (Caco-2,HT29-MTX),以测量IL-8的表达.
主要成果:
- 来自ETBF和NTBF的OMV含有丰富的编码和非编码RNA,特定RNA (BF9343_RS22680,BF9343_RS17870) 在ETBFOMV中显著高.
- OMVs激活了TLRs2,3和7并增加了肠细胞中的IL-8表达.
- 在ETBFOMV中的RNA降解,但不是NTBFOMV,增强了IL-8刺激,表明这些extravesicularRNAs具有免疫抑制作用.
结论:
- 与OMV相关的RNA在宿主免疫调节中起着双重作用,其中一些具有免疫抑制作用.
- 这些发现对了解细菌病原和开发新型治疗策略具有重要意义.
- 来自致病菌与共生菌株的OMV的差异性RNA含量和功能突出了与宿主免疫系统的菌株特异性相互作用.
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