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相关概念视频

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

2.7K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
2.7K
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

1.2K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.2K
Precipitation Processes01:12

Precipitation Processes

604
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
604
X-ray Crystallography02:18

X-ray Crystallography

24.2K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
24.2K
Quality of Water01:19

Quality of Water

194
In concrete preparation, the quality of water is paramount as it affects the strength and durability of the concrete. Potable water is usually preferred; however, it must not have excessive sodium or potassium to prevent compromising the concrete's integrity. Water quality is typically evaluated based on impurities such as dissolved solids, chlorides, and sulfates, and its pH value is ideally between 6 and 8. Even slightly acidic natural water may be acceptable unless it contains harmful...
194
Types of Coprecipitation01:10

Types of Coprecipitation

886
Coprecipitation is the contamination of a precipitate by otherwise soluble species and occurs via different processes. In colloidal precipitates, coprecipitation occurs via surface adsorption. For instance, barium sulfate has a primary layer of adsorbed barium ions and a secondary layer of nitrate counterions. This results in contamination of the precipitate by barium nitrate.
Sometimes, ions in a crystal lattice can undergo isomorphous replacement by inclusions of similar charge and size. For...
886

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On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
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跨越池会影响水晶质量吗?

Christopher S Campomizzi1,2, M Elizabeth Snell1,2, Halina Mikolajek3,4

  • 1University at Buffalo Hauptman Woodward Institute, 700 Ellicott Street, Buffalo, NY, 14203, United States.

bioRxiv : the preprint server for biology
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PubMed
概括
此摘要是机器生成的。

在室温下运送蛋白质晶体 (RT) 用于X射线衍射是可行的. 长途运输不会损害晶体的完整性或数据质量,从而使RT结晶学获得更广泛的访问.

关键词:
室温结晶学 室温结晶学在现场的X射线衍射.蛋白质晶体的运输 运输

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科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 晶体学 晶体学是指结晶学.

背景情况:

  • 室温 (RT) X射线衍射对于研究蛋白质动态和碎片结合至关重要.
  • 现场多功能宏分子结晶学 (VMXi) 束线可直接从结晶板收集RT数据,从而保持晶体的完整性.
  • 对于运输冷晶体存在既定的协议,但运输RT晶体的影响仍然未经检查.

研究的目的:

  • 研究远程运输对用于RT X射线衍射的蛋白质晶体的影响.
  • 为了确定环境温度运输是否会损害晶体完整性和衍射数据质量.

主要方法:

  • 蛋白质晶体是在布法罗大学霍普特曼伍德沃德研究所 (UB-HWI) 的MiTeGen In Situ-1板上生长的.
  • 结晶板通过跨大西洋运送到英国的钻石光源 (DLS),使用斯坦福同步辐射光源 (SSRL) 蓝盒热发货器.
  • 在VMXi光线上收集了运输和本地准备的晶体的RT X射线衍射数据.

主要成果:

  • 在运输的RT晶体和现场准备的晶体之间没有观察到数据质量的显著差异.
  • 从运送的晶体中成功确定了高分辨率的蛋白质结构.
  • 长途运输RT晶体并没有对衍射质量产生负面影响.

结论:

  • 在环境温度下长途运输蛋白质晶体是RT X射线衍射的可行方法.
  • 这种工作流扩大了全球对RT结晶学技术的访问,减少了对现场结晶生长的需求.
  • 世界各地的研究人员可以在没有地理限制的情况下利用先进的RT结晶学.