单核转录组学揭示了细胞类型如何塑造阿拉比多普西斯叶的循环转录组
Alveena Zulfiqar1,2, Zachary A Myers1,2, Ananda Menon1
1Department of Plant and Microbial Biology, University of Minnesota, St. Paul, MN 55108, USA.
bioRxiv : the preprint server for biology
|July 16, 2025
概括
植物使用昼夜调节来协调日常周期,但细胞特异性的时钟动力学尚不清楚. 这项研究揭示了Arabidopsis叶细胞类型中广泛的昼夜基因调节,具有复杂的,细胞特异性网络.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 循环节律对于植物适应日常环境变化至关重要.
- 对成熟植物叶子中细胞类型特定的昼夜调节的理解是有限的.
研究的目的:
- 为了研究成熟的Arabidopsis thaliana叶子中不同细胞类型的昼夜基因调节的动态和层次.
- 识别特定于细胞类型的时间表达模式和调节网络.
主要方法:
- 在Arabidopsis thaliana的叶子上进行了24小时的单核RNA测序昼夜时间过程.
- 在七个时间点捕获了大约3万个细胞核,涵盖了所有主要的叶子细胞类型.
- 利用协同表达分析和GENIE3来定义时间表达分类并生成单细胞基因调控网络.
主要成果:
- 鉴定了超过7,400个具有集群解决的昼夜调节的基因,组织成五个主要的时间表达分支,跨细胞类型共享.
- 发现了基因表达中的细胞类型特定的时间转移,并确定了许多共享和独特的转录因子相互作用.
- 发现了与核心时钟基因目标相关的复杂光和激素信号网络,显示了广泛和细胞类型特定的调节.
结论:
- 循环转录调节在成熟的阿拉比多普西斯叶子中是广泛的.
- 细胞类型特定的昼夜调节存在显著的复杂性,涉及复杂的基因网络和信号通路.
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