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Updated: Sep 15, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

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使用线性DNA捐赠者的原始组装使得大型基因组插入成为可能.

Bin Liu1, Andrew Petti1, Xuntao Zhou1

  • 1RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, USA.

bioRxiv : the preprint server for biology
|July 16, 2025
PubMed
概括

主编组件 (PA) 能够为基因组工程进行大规模的DNA插入,克服双主编辑 (PE) 的局限性. 这种方法有效地使用重叠模板和NHEJ抑制剂插入大型DNA片段,避免双链DNA断裂.

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The donor site from where the transposon is excised is either degraded or...
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