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In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Updated: Jun 16, 2026

Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

Published on: December 27, 2013

投影特异性交叉光遗传学用于马尔莫塞特大脑中的精确激发和抑制.

Luke Shaw¹, Krishnan Padmanabhan¹, Amy Buckleaw¹

¹Department of Neuroscience, Del Monte Institute for Neuroscience, University of Rochester Medical Center, Rochester, NY 14642, USA.

bioRxiv : the preprint server for biology

|July 16, 2025

概括

研究人员开发了一种新的光遗传学方法,以精确控制小熊猫中的特定神经元路径. 这种工具允许有针对性的激发和抑制,推进对大脑电路和高阶功能的研究.

关键词:

克雷复合酶可以重组.一个Dlx增强器.交叉视觉遗传学交叉视觉遗传学腺相关病毒的病毒.激发和抑制的激发和抑制.层层记录层层记录马尔莫塞特人马尔莫塞特人预测特定的投影.反向标签的标签是反向的

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Stereotaxic Surgery for Implantation of Microelectrode Arrays in the Common Marmoset Callithrix jacchus

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Published on: September 29, 2019

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Published on: February 25, 2022

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Last Updated: Jun 16, 2026

Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits

Published on: December 27, 2013

Stereotaxic Surgery for Implantation of Microelectrode Arrays in the Common Marmoset Callithrix jacchus

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Published on: September 29, 2019

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Published on: February 25, 2022

科学领域:

神经科学是一个神经科学.
灵长类动物研究研究.
视觉遗传学视觉遗传学

背景情况:

灵长类大脑皮层使用远程连接进行信息整合.
对于调节这些路径的现有工具是有限的.
了解这些电路对于研究高阶大脑功能至关重要.

研究的目的:

开发一个优化的光遗传策略,精确调制鱼中的投射特异性神经元.
为了实现神经通路的选择性激发和抑制.
扩大灵长类大脑功能因果研究工具包.

主要方法:

使用了交叉病毒和光遗传学的策略.
调整了一个从小鼠到大黄蜂的管道.
使用Cre-复合酶 (AAVretro-Cre) 的逆向输送和局部注射的Cre-依赖的素载体 (AAV8-FLEx-ChR2或下巴).
使用AAV9-Dlx-ChR2.2验证的局部皮质抑制.

主要成果:

达到了指向选择性表达在骨和前双通道.
通过使用双素共同表达,证明了精确的体内刺激或抑制投射神经元.
观察到最小的目标外标标签.
证实了强大的局部皮质抑制.

结论:

建立了一个可扩展的框架,用于对灵长类动物电路的投影特异性光遗传质疑.
在实验工具中显著提高了解剖学精度和功能特异性.
提供了一种强大的新方法,用于对灵长类动物大脑功能的因果调查.