Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Organization of Genes02:07

Organization of Genes

69.6K
Overview
69.6K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

12.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.3K
RNA Splicing01:32

RNA Splicing

57.1K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.1K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

47.7K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
47.7K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.9K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.9K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

6.8K
6.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes.

eLife·2026
Same author

Functional requirement for Dicer helicase arginine methylation in 26 G siRNA biogenesis and oocyte meiotic program.

Nature communications·2026
Same author

A eukaryote without tRNA introns.

RNA (New York, N.Y.)·2025
Same author

Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes.

bioRxiv : the preprint server for biology·2025
Same author

The highly conserved intron of tyrosine tRNA is critical for <sup>m1</sup>A58 modification and controls the integrated stress response.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies.

G3 (Bethesda, Md.)·2025
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Sep 15, 2025

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

21.7K

一个没有tRNA内核的真核生物.

Ambro Van Hoof, Tokiko Furuta, Swathi Arur

    bioRxiv : the preprint server for biology
    |July 16, 2025
    PubMed
    概括
    此摘要是机器生成的。

    一些真核生物缺乏tRNA内子,挑战它们的普遍性. 一种线虫物种,Levipalatum texanum,完全失去了tRNA内核及其tRNA内核酶,为内核进化和拼接功能提供了洞察力.

    更多相关视频

    Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
    11:19

    Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

    Published on: February 25, 2011

    20.0K
    A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
    06:48

    A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

    Published on: June 16, 2022

    2.1K

    相关实验视频

    Last Updated: Sep 15, 2025

    Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
    13:16

    Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

    Published on: January 22, 2018

    21.7K
    Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
    11:19

    Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

    Published on: February 25, 2011

    20.0K
    A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
    06:48

    A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

    Published on: June 16, 2022

    2.1K

    科学领域:

    • 基因组学就是基因组学.
    • 分子生物学分子生物学
    • 进化生物学 进化生物学

    背景情况:

    • 细胞基因组包含结合体,XBP1mRNA和tRNA的内子.
    • 在真核生物中,tRNA 内核和tRNA 内核酶被认为是普遍的.
    • 在Tyr-GUA,Ile-UAU和Leu-CAA tRNA中的内子在Metazoa中保存.

    研究的目的:

    • 研究真核细胞中tRNA内核的进化损失.
    • 描述一个缺少tRNA内核的线虫物种.
    • 了解对tRNA拼接和修饰酶的影响.

    主要方法:

    • 对线虫物种进行比较基因组学分析.
    • 在特定的tRNA中识别内子存在/不存在.
    • 分析相关的遗传变化,包括基因配对和酶存在.

    主要成果:

    • 一些线虫在Tyr-GUA和Ile-UAU的tRNA中失去了内部子.
    • 莱维帕拉图姆 (Levipalatum texanum) 完全没有tRNA内核和tRNA内核酶.
    • L. texanum表现出改变的tRNA抗杆茎环和tRNA脱氨酶基因重复.
    • L. texanum保留了tRNA结合酶和XBP1内部.

    结论:

    • 在所有真核生物中,tRNA 内子并非普遍保留.
    • 丢失的tRNA内核可以伴随着适应性遗传变化.
    • L. texanum为独立研究tRNA拼接和修改途径提供了一个独特的模型.