一种系统生物学方法来评估女性阴道健康的潜在益生菌候选人
bioRxiv : the preprint server for biology
|July 16, 2025
概括
大多数益生菌补充剂缺乏对物种选择的明确功能基础. 这项研究开发了一种代谢建模框架,揭示了当前的益生菌错过了原生微生物的代谢多样性,并表明D-乳酸盐的产生是抑制阴道病原体的关键机制.
科学领域:
- 微生物学 微生物学
- 系统生物学 系统生物学
- 计算生物学 计算生物学
背景情况:
- 益生菌补充剂被广泛推销为健康益处,但物种选择往往缺乏功能上的理由.
- 对352种美国益生菌产品的调查显示,微生物多样性有限,物种与声称的健康益处之间没有明确的联系.
研究的目的:
- 用基因组规模的代谢模型评估益生菌物种的功能能力.
- 通过比较代谢特征与病原体Gardnerella vaginalis,确定有利于阴道健康的微生物物种.
- 为基于功能的益生菌设计建立一个可扩展的框架.
主要方法:
- 开发了CoPaPro,这是对各种细菌的1,012个基因组规模代谢模型的集合.
- 利用流量平衡分析来评估益生菌物种的代谢多样性和功能.
- 用阴道分离物进行了体外消耗介质测定,以评估G. vaginalis.的抑制.
主要成果:
- 目前的益生菌物种与本地食人相比,表现出有限的代谢多样性.
- 确定了具有代谢概况重叠的共生细菌G. vaginalis.
- 实验室试验表明,D-乳酸的产生,而不仅仅是代谢相似性,是抑制G. vaginalis的关键,几种非Lactobacillus物种显示出有效性.
结论:
- 益生菌的设计应该优先考虑功能上的理由,而不是任意的物种.
- 与实验验证相结合的代谢建模为发现和设计有效益生菌提供了强大的方法.
- D-乳酸的产生是G. vaginalis的益生菌抑制的一个重要机制,突出了潜在的非乳杆菌候选人.
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