相关实验视频

Updated: Sep 15, 2025

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

酸身份调节宿主热带性糖结合维里丹群链球菌群

KeAndreya M Morrison¹, Rupesh Agarwal², Haley E Stubbs³

¹Department of Pharmacology, School of Graduate Studies, Meharry Medical College, Nashville, TN, USA.

bioRxiv : the preprint server for biology

|July 16, 2025

概括

病毒群链球菌与酸结合 (Neu5Ac和Neu5Gc) 影响宿主特异性. 修改结合偏好改变了细菌宿主目标,但单独的酸类型不能完全确定宿主热带性.

科学领域:

微生物学微生物学
葡萄糖生物学葡萄糖生物学
宿主-病原体相互作用

背景情况:

微生物与各种宿主之间的相互作用可以创建病原体储存库,并促进动物传染的溢出.
维里丹斯群链球菌与宿主糖体结合,这些糖体被终端酸覆盖.
人类的酸是N-乙神经胺酸 (Neu5Ac),而哺乳动物也合成N-甘醇神经胺酸 (Neu5Gc).

研究的目的:

通过实验测试酸结合偏好与链球菌宿主偏好之间的联系.
调查酸标识 (Neu5Ac与Neu5Gc) 在确定链球菌宿主热带性中的作用.

主要方法:

研究了来自*Streptococcus gordonii* (SLBR_Hsa) 和*Streptococcus sanguinis* (SLBR_SrpA) 的Siglec类结合区域 (SLBR) 的糖结合特性.
使用结构和计算分析来了解Neu5Ac/Neu5Gc结合.
为改变对Neu5Gc终结型甘氨酸的选择性而设计的SLBR,并测试了与人类和老鼠血受体的结合.

主要成果:

结构分析揭示了Neu5Ac或Neu5Gc封顶的二糖结合的分子细节.
为Neu5Gc选择性进行工程,将结合从人类转移到大鼠血受体.
然而,偏好纯化酸覆盖的甘氨酸并不能完全预测宿主受体偏好.

结论:

酸的身份调节,但不仅仅决定,宿主偏好在这些链球菌.
这些发现挑战了假设酸类型是宿主热带主义的主要驱动因素的假设.
这项研究完善了对细菌宿主特异性背后的机制的理解.

关键词:

西格莱克类型的绑定区域.粘附在其中的粘合物.这是一种共生主义 (commensalism).交叉反应性交叉反应性主机偏好的主机偏好.主体受体受体的宿主受体是什么传染性内心炎是一种感染性内心炎.口腔微生物组是口腔的微生物组.病变的发生和发病.酸是酸的一种.链球菌是一种链球菌.

更多相关视频

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines

Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines

Published on: November 25, 2017

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Published on: July 6, 2016

相关概念视频

Glycocalyx and its Functions

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...