抗生素在哺乳动物细胞中重新连接核心代谢和核糖体程序
Cameron S Movassaghi1,2,3, Jesse G Meyer1,2,3
1Department of Computational Biomedicine, Cedars Sinai Medical Center, Los Angeles CA 90048.
bioRxiv : the preprint server for biology
|July 16, 2025
概括
哺乳动物细胞培养中的抗生素使用会影响蛋白质水平,影响实验结果. 这项蛋白质组学研究揭示了非目标效应,并为设计细胞培养实验提供了见解.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 细胞生物学 细胞生物学
- 哺乳动物细胞培养
背景情况:
- 抗生素通常用于哺乳动物细胞培养,以防止细菌污染.
- 抗生素对细胞培养蛋白质的影响没有得到很好的描述,与基因组和转录组效应不同.
- 之前的研究已经记录了抗生素治疗细胞中的基因组和转录组差异.
研究的目的:
- 进行一个全面的哺乳动物细胞培养和没有抗生素 (青素/链杆菌素) 的蛋白质组比较.
- 研究抗生素对HepG2细胞蛋白质的非向作用.
- 分析抗生素治疗对细胞蛋白质组概况的纵向影响.
主要方法:
- 纵向和交叉研究设计,分析了9个通道和4个条件的119个样本.
- 使用质谱学的蛋白质组分析来量化蛋白质丰富度.
- 线性混合效应建模,以评估抗生素治疗的时间效应.
主要成果:
- 在接受抗生素治疗和未接受治疗的HepG2细胞之间鉴定出383种不同丰富的蛋白质.
- 观察到核糖体和线粒体蛋白质的变化,表明蛋白质层面的非目标效应.
- 蛋白质影响在第一个通道最强,在第三个通道稳定;启动治疗比停止治疗有更大的影响.
结论:
- 哺乳动物细胞培养中的抗生素治疗诱导了显著的,保存的蛋白质变化.
- 这些发现强调了在实验设计和数据解释中考虑抗生素效应的重要性.
- 这项研究为研究人员使用抗生素治疗的细胞模型提供了宝贵的蛋白质基因资源.
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