在患有或没有1型糖尿病的儿童中,岛屿自身免疫性血清转换时的免疫细胞概况的差异:岛屿自身免疫性周围的免疫细胞变化
Kirk R Hohsfield1,2, Patrick M Carry1,2,3, Sarah D Slack2
1Department of Epidemiology, Colorado School of Public Health, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
bioRxiv : the preprint server for biology
|July 16, 2025
概括
早期的免疫细胞转移先于1型糖尿病的发展. 中性粒细胞与淋巴细胞比率 (NLR) 和T细胞比率的变化表明,在后来发展为1型糖尿病的个体中,免疫成熟发生了变化.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 遗传学 是一个遗传学.
背景情况:
- 血清转化 (SV) 表示小岛自身免疫 (IA) 的开始,即1型糖尿病 (T1D) 的临床前阶段.
- 免疫细胞 (不包括细胞毒性T细胞) 在IA启动中的作用尚未完全理解.
- 了解早期的免疫动态对于T1D病变研究至关重要.
研究的目的:
- 在T1D发育中的血清转化前和期间调查免疫细胞类型比例和功能比率.
- 为了确定T1D病例和对照人群之间的免疫细胞动态的差异.
- 探索免疫细胞比率作为T1D风险分层的潜在生物标志物.
主要方法:
- 应用了来自外周血液的DNA甲基化数据的高分辨率免疫细胞类型解.
- 分析了DAISY和TEDDY研究中的嵌套病例控制样本 (n=317).
- 利用线性模型评估在SV前和SV时间点上的免疫细胞比例和比率.
主要成果:
- 在SV之前,T1D病例与对照人群相比,具有较高的中性粒细胞与淋巴细胞比率 (NLR) 和较低的CD4T/CD8T细胞比率.
- 在SV时,病例显示B-CD4T-CD8T组合记忆/原始细胞比率降低.
- 从前-SV到SV,病例显示了减弱的NLR,B-记忆/先天和B-CD4T-CD8T记忆/先天的比率.
结论:
- 在T1D发病过程中观察到先天性和适应性免疫细胞动态的早期转变.
- 这些模式表明免疫成熟的延迟或中断,可能涉及持久的原始细胞或受损的记忆过渡.
- 免疫细胞比率可以作为早期T1D风险评估和了解疾病机制的宝贵生物标志物.
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