相关实验视频

Updated: May 7, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

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阿克罗莱因作为一种基于活动的替代性广泛反应性探针,用于基于活动的蛋白质剖析囊蛋白组

Jiahua Zhou1,2, Kailu Tian1, Yanan Li1

  • 1State Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, Biotechnology Division, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Analytical chemistry
|July 16, 2025
PubMed
概括

一种名为acrolein (ACR) 的新探针通过与传统探针相比,与更多的核性残留物反应,扩大了基于活动的蛋白质概况 (ABPP). 这种多功能工具有助于识别功能性核性位点,并且在药物发现方面具有潜力.

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Detection of Protein S-Acylation using Acyl-Resin Assisted Capture08:31

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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