一种基于深度浸泡溶剂的绿色消化方法,用于确定结核病儿童血清样本中的必需微量元素
Aijaz Ahmed Memon1, Tasneem Gul Kazi1, Mohammad Nur-E-Alam2
1National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan, postal code 76080.
Journal of AOAC International
|July 16, 2025
概括
儿童结核病患者表现出铁和等微量元素的水平发生变化. 治疗使这些水平正常化,表明它们在免疫功能和疾病恢复中的作用.
科学领域:
- 生物化学 生物化学
- 儿童传染病 儿童传染病
- 环境化学环境化学
背景情况:
- 结核病 (TB) 是一个全球性的健康威胁,特别影响发展中国家的儿童.
- 肺结核的免疫力受损与改变的微量元素基本度有关.
研究的目的:
- 为了确定血清铜 (Cu),铁 (Fe) 和 (Zn) 水平的变化,在患有肺结核 (PTB) 的5-10岁儿童中.
- 评估六个月的抗结核治疗对这些微量元素度的影响.
主要方法:
- 血清样本使用环保的基于溶剂的深度环氧化消化方法进行了分析.
- 诱导合等离子体原子辐射光谱法用于量化Cu,Fe和Zn.
- 优化了消化参数,包括溶剂成分,温度和超声波时间.
主要成果:
- 与健康对照组相比,患有PTB的儿童的血清Fe和Zn水平显著降低,Cu/Zn比率更高.
- 这些变化可能与活跃的疾病有关,而不是初级缺陷.
结论:
- 六个月的抗结核治疗导致血清Fe (16%) 和Zn (30%) 显著增加,Cu (23%) 减少.
- 处理后的水平接近,但仍略低于参考值.
更多相关视频
09:57System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
8.7K
09:54Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
Published on: February 16, 2020
5.8K
相关概念视频
Pulmonary Tuberculosis IV
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Serum Laboratory Studies, Stool Test, Breath Test
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
