在SARS-CoV-2编码的ORF3a与YY1相互作用,促进潜伏的HCMV重新激活
Sisi Xia1,2, Xiaoping Huo2,3, Nanfang Liu3
1Department of Biological Engineering, Wuhan Polytechnic University, Wuhan, China.
PLoS pathogens
|July 16, 2025
概括
严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 激活了人类细胞巨化病毒 (HCMV) 的重新激活. 在SARS-CoV-2的ORF3a蛋白针对阴阳1 (YY1),促进病毒复制和提供新的治疗策略.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 人类细胞巨乳病毒 (HCMV) 建立了终身潜伏期,其活性由髓状细胞中病毒主要即时早期促进体 (MIEP) 的染色质结构调节.
- 细胞和病毒因素影响HCMV的重新激活.
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 已被观察到促进HCMV的重新激活,但根本机制尚未完全理解.
研究的目的:
- 阐明SARS-CoV-2促进HCMV重新激活的分子机制.
- 为了确定SARS-CoV-2涉及HCMV重新激活的特定病毒因子.
- 研究SARS-CoV-2与调节HCMV延迟的细胞因子之间的相互作用.
主要方法:
- 研究了SARS-CoV-2 ORF3a和阴阳1 (YY1) 之间的相互作用.
- 评估了SARS-CoV-2 ORF3a对YY1稳定性和无处不在性的影响.
- 研究了这种相互作用对HCMV MIEP激活,病毒再激活,复制和扩散的影响.
主要成果:
- SARS-CoV-2 ORF3a与YY1直接相互作用,YY1是已知的HCMV MIEP的抑制剂.
- 这种相互作用导致YY1.1的依赖于乌比奎的降解.
- YY1的降解导致HCMV MIEP的激活,促进HCMV的重新激活,复制和扩散.
结论:
- SARS-CoV-2的ORF3a蛋白质通过向YY1进行降解,在促进HCMV的活性化方面发挥着关键作用.
- 这项研究揭示了在联合感染期间由SARS-CoV-2介导的HCMV重新激活的分子机制.
- 这些发现为开发针对SARS-CoV-2和HCMV联合感染的治疗策略提供了理论基础.
更多相关视频
13:22Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
Published on: January 23, 2014
18.4K
16:49Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Published on: July 16, 2012
11.7K
相关概念视频
Rous Sarcoma Virus (RSV) and Cancer
5.4K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.4K
Viral Recombination
23.8K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.8K
Exon Recombination
3.7K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.7K
Size and Structure of Viral Genomes
158
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
158
Immune Response Against Viral Pathogens
929
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
929
Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
