流感A病毒核蛋白 (NP) 基因中具有优化/脱优化的同名编码子对NP和三方基因蛋白25 (TRIM25) 之间的相互作用的影响
Xinyan Hu1, Mingyang Gao1, Xiaoting Ren1
1Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China; College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu, 730010, China.
Virology
|July 16, 2025
概括
在A型流感病毒 (IAV) 中同义代码偏差核蛋白 (NP) 影响mRNA稳定性和TRIM25相互作用. 优化编码子会损害NP功能,解释进化编码子使用模式.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 核蛋白 (NP) 对A型流感病毒 (IAV) 生命周期至关重要.
- 尽管存在一些变化,但IAV NP表现出显著的同义代码子使用偏差.
研究的目的:
- 调查同名编码子使用对IAV NP mRNA稳定性,翻译效率和TRIM25相互作用的影响.
- 了解推动IAV NP中的子偏差的进化压力.
主要方法:
- 构建NP突变 (NPoptimal,NPrare) 通过用有利的或罕见的同名编码子替换编码子.
- 评估野生类型和突变NP的mRNA稳定性和翻译效率.
- 评估了NP变体和三方基因蛋白25 (TRIM25) 之间的相互作用.
主要成果:
- 与野生型NP相比,NPoptimal和NPrare突变都显示出mRNA稳定性和翻译效率受损,NPoptimal更受影响.
- NPoptimal完全丧失了对TRIM25的结合能力,而NPrare则显示结合能力明显受损.
- 同义代码子的变化,特别是在NP的前期部分,损害了它与TRIM25的相互作用.
结论:
- 同义代码子的使用显著影响IAV NP mRNA的稳定性,翻译和与TRIM25的相互作用.
- 这项研究提供了关于IAV NP为什么保持强烈的同义符号使用偏差的见解.
- 了解这些代级影响,可以揭示IAV NP的进化机制.
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