协同生物机械预处理和料批量半SSF战略,用于从拉米草中增强纤维素乙醇生产
Wei Yang1, Yuanyuan Deng2, Zhaoli Wang2
1Hengyang Normal University, Hengyang 421200, China.
International journal of biological macromolecules
|July 16, 2025
概括
这项研究提出了一种新的机械生物预处理和料批发发酵策略,用于从拉米草有效生产乙醇. 该过程实现了高葡萄糖产量和显著的乙醇转化率,提供了可持续的生物炼制解决方案.
科学领域:
- 生物技术是生物技术.
- 生物精炼是生物精炼的一种方式.
- 可持续化学 可持续化学
背景情况:
- 红纤维素生物质的再生阻碍了有效的生物燃料生产.
- 拉米提供了一个未充分利用的纤维素资源.
- 目前的预处理方法往往涉及强化学品,影响可持续性.
研究的目的:
- 开发一个完整的机械生物预处理策略,用于拉米草.
- 优化料批量高固体半同时糖化和发酵 (S-SSF) 过程以生产乙醇.
- 为了实现有效和可持续的乙醇转化从拉米草没有有害化学物质.
主要方法:
- 双重的真菌预处理 (Pleurotus ostreatus和Ganoderma lucidum) 用于素和半纤维素的去除.
- 球磨,以提高酶的消化能力.
- 在25%的固体中以索福利皮德辅助的料批次酶性水解.
- 在72小时的预水解后,半同时的糖化和发酵 (S-SSF).
主要成果:
- 通过真菌预处理选择性去除木质素 (59.8%) 和半纤维素 (41.0%).
- 球磨机将葡萄糖产量提高到71.7% (增加了5.48倍).
- 料批量水解实现了88.7 g/L的葡萄糖标位.
- S-SSF产生了43.0g/L的乙醇,其转换率为115g/kg的拉米.
结论:
- 机械生物预处理和料批量S-SSF战略的结合有效地提高了从拉米草中生产乙醇的产量.
- 这种环保的方法为传统的生物提炼方法提供了一个可持续的替代方案.
- 该过程表明,在工业规模上利用基纤维素生物质的巨大潜力.
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