由Cupriavidus necator DSM 545从乳清透酸中生产的可持续的多基酸盐
Marina Basaglia1, Sergio Casella1, Giuliana Franzosi2
1Waste to Bioproducts Lab, DAFNAE - Department of Agronomy Food Natural Resources Animals and Environment, Viale dell'Università 16, 35020 Legnaro, Padova, Italy.
International journal of biological macromolecules
|July 16, 2025
概括
用β-银酸酶酶处理乳清透酸使Cupriavidus necator能够有效地产生多氧酸盐 (PHAs). 这种可持续的方法提高了乳制品行业副产品的生物聚合物产量.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 环境科学 环境科学
背景情况:
- 聚酸酸 (PHAs) 提供了可生物降解的塑料替代品,但受到高生产成本的限制.
- 乳清透酸是一种富含乳糖的乳制品副产品,是生物聚合物合成的可持续碳来源.
- 常见的PHA生产者Cupriavidus necator不能直接利用乳糖,阻碍其与乳制品残留物的使用.
研究的目的:
- 为了研究乳清透中的乳糖的酶性水解,以提高Cupriavidus necator的PHA生产.
- 评估使用经过处理的乳清透物作为PHA生物合成的碳基质的可行性.
- 将经过处理的乳清透的PHA产量与纯糖的PHA产量进行比较,并探索同时糖化和发酵 (SSF) 方法.
主要方法:
- 使用Maxilact® LGI 5000 (β-galactosidase) 在乳清透中的乳糖的酶性水解.
- 培养Cupriavidus necator DSM 545 在最小介质中,用水解乳清透剂进行培养.
- 优化酶状况,评估生物质和PHA积累.
- 实施一次同时的糖化和发酵 (SSF) 过程.
主要成果:
- 水解乳清透物支持了C. necator的生长,达到超过5g/L的生物质度.
- PHA积累达到细胞干重量的约60%,相当于纯糖基质.
- SSF的设置简化了生物转化过程,表明了其工业应用的潜力.
- 收益率与使用常规糖基介质获得的收益率相当或高于收益率.
结论:
- 用β-银酸酶对乳清透的酶性处理有效地使Cupriavidus necator能够产生PHA.
- 使用经过处理的乳清透剂为PHA生物合成提供了具有成本效益和可持续性的途径.
- 建议进一步优化工艺,但结果表明,乳制品残留物可用于生物塑料的价值化具有重大潜力.
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