伴侣诱导的DNA复制因子小染色体维护蛋白10促进了Plutella xylostella中的 oogenesis
Lu Peng1, Laiwai Tun2, Yurui Qian1
1State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; International Joint Research Laboratory of Ecological Pest Control of Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Youxi-Yangzhong Vegetable Pest Prevention and Control, Fujian Observation and Research Station, Fuzhou 350002, China.
交配触发了雌性Plutella xylostella细胞周期的恢复,MCM10蛋白对DNA复制和卵细胞发育至关重要. 这一发现为新的害虫管理策略提供了潜力.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 产卵发生涉及细胞循环停止和恢复交配后,对于昆虫繁殖至关重要.
- 在Plutella xylostella中 oogenesis 的分子机制尚不清楚.
- 交配会诱导雌性昆虫显著的基因表达变化.
研究的目的:
- 为了阐明调节Plutella xylostella在交配后的 oogenesis 的分子机制.
- 识别参与DNA复制和卵巢发育的关键基因.
- 探索潜在的害虫控制目标.
主要方法:
- 对比转录组学分析以确定差异表达的基因.
- 小染色体维护 (MCM) 基因的家族遗传学分析.
- 定量实时PCR (qRT-PCR) 用于基因表达分析.
- 基因淘汰和功能分析的RNA干扰 (RNAi).
主要成果:
- 交配诱导了1200个不同表达的基因,包括MCM10的上调.
- PxMCM10在成年女性的卵巢和卵子中特别表达.
- PxMCM10敲击破坏了DNA复制,卵细胞发育和胆管形成,同时增加了亡.
- 在PxMCM10敲击后观察到的维特洛金和胆相关基因的下调.
结论:
- PxMCM10在P. xylostella.交配后的DNA复制重启调节中发挥着至关重要的作用.
- PxMCM10 影响毛囊细胞功能,影响生和胆管形成.
- PxMCM10是开发类害虫遗传害虫管理策略的潜在目标.
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