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加快生物疗法发现:通用LC-MALDI-MS离线方法的蓝图

Elizabeth F Bayne¹, Xiong-Zhuo Gao¹, Yuan Jiang¹

¹Discovery Analytical Research, Analytical R&D, MRL, Merck & Co., Inc., Boston, MA, 02115, USA.

Analytica chimica acta

|July 16, 2025

概括

一个新的自动化液体染色学-矩阵辅助激光消耗电离-质谱学 (LC-MALDI-MS) 工作流程可以快速识别生物治疗杂质. 这种方法克服了SEC和HIC等技术的电喷射电离 (ESI) 的局限性,加速了药物发现.

科学领域:

生物制药学分析分析
分析化学是一种分析化学.
质谱测量质量谱测量

背景情况:

生物治疗对治疗各种疾病至关重要.
高通量液体色谱 (LC) 对于生物治疗发现至关重要.
传统的LC方法面临着质谱 (MS) 兼容性的挑战,减缓了杂质的识别.

研究的目的:

开发一个快速和自动化的工作流程,用于生物治疗峰值的识别.
为了克服MS的兼容性问题与常见的LC技术.
为了加速下一代生物学的发现和发展.

主要方法:

开发了一个离线LC-MALDI-MS工作流,使用LC-分数收集器和MALDI-TOF-MS.
该方法与各种LC技术兼容,包括尺寸排除色谱 (SEC),疏水性相互作用色谱 (HIC) 和逆相LC.
工作流被设计为自动化和最小的样本准备.

主要成果:

在几个小时内实现了快速峰值识别.
在不同的染色学方法和分析物 (抗体,融合蛋白,) 中展示了多功能性.
克服缓冲器和矩阵干扰,通常在电喷离子化 (ESI-MS) 中见到.

结论:

自动化的LC-MALDI-MS工作流显著简化了生物治疗杂质的识别.
这种方法通过快速分析来加快药物发现时间表.
工作流程广泛适用于生物制药开发的各个阶段.

关键词:

抗体与药物的结合物.生物治疗学生物治疗学双特异性抗体双特异性抗体 LC 分数收集器分数收集器马尔迪-托夫 (MALDI-TOF) 是一个国家元首.酸是一种酸.

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相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...