用响应表面方法学选和表征土壤细菌的红素和织染料废水生物修复和优化
Sarah Milad1, Sarra E Saleh1, Mohammad M Aboulwafa2,3
1Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Scientific reports
|July 16, 2025
概括
这项研究确定了埃及土壤中的Streptomyces intermedius 434作为脱色织染料的强有力的细菌候选者. 优化条件显著增强了其化活性,由生物修复应用的全基因组测序证实.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 工业染料废水带来了严重的环境污染挑战.
- 使用微生物剂进行生物修复为染料脱色提供了一个可持续的解决方案.
- 识别具有强烈的微生物,具有线性溶解能力,对于有效的废水处理至关重要.
研究的目的:
- 隔离和识别能够去色各种工业染料的细菌菌株.
- 使用Streptomyces intermedius优化染料脱色过程.
- 通过全基因组测序来研究线性溶解和染料脱色活动的遗传基础.
主要方法:
- 从埃及农业土壤中分离和选177种细菌菌株.
- 使用Azure B,甲基蓝和刚果红的染料脱色试验.
- 16S rRNA测序用于细菌识别.
- 使用一次性一个因素 (OFAT) 和响应表面方法 (RSM) 优化脱色条件.
- 选择的隔离体的全基因组测序 (WGS).
主要成果:
- 两种分离物,分别称为灰色红色菌和中色菌,显示出高的脱色能力.
- 对Streptomyces intermedius 434条件的优化导致染料脱色效率增加了2.6倍.
- 全基因组测序揭示了编码酶的基因,这些酶对素降解和染料脱色至关重要.
- 工业地质学数据与观察到的表型染料脱色活性相关.
结论:
- 菌株Streptomyces intermedius 434是织物染料废水生物修复的一个有希望的候选物.
- 鉴定到的解酶和优化条件提高了脱色效率.
- 全基因组测序为了解和改善微生物染料降解能力提供了遗传基础.
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