综合性基因组重建揭示了人类肠道双菌中碳水化合物利用的异质性
Aleksandr A Arzamasov1, Dmitry A Rodionov1, Matthew C Hibberd2,3
1Center for Data Sciences, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.
Nature microbiology
|July 16, 2025
概括
双菌的新陈代谢因菌株而异,影响益生菌的有效性. 这项研究绘制了它们的碳水化合物利用途径,揭示了针对定制共生设计的显著功能多样性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 代谢工程是代谢工程.
背景情况:
- 双杆菌是重要的糖溶性微生物,用于益生菌和共生菌.
- 对Bifidobacteria补充剂的个人反应因菌株类型,宿主微生物群,饮食和生活方式而异.
- 对于有效的益生菌和共生菌配方,需要对甘氨酸代谢的菌株特定见解.
研究的目的:
- 重建和分析Bifidobacteria的代谢途径,以利用碳水化合物.
- 调查跨Bifidobacterium物种和菌株的糖代谢中的功能异质性.
- 为设计向益生菌和共生菌配方提供基因组综合资料.
主要方法:
- 通过分析3,083个Bifidobacterium基因组中的589个代谢基因功能来重建68个甘氨酸利用途径.
- 在实验室验证了38个预测的表型,用于30个地理上不同的Bifidobacterium菌株.
- 基因组分析以确定碳水化合物代谢中的物种间和物种内功能变异.
主要成果:
- 在Bifidobacteria的碳水化合物代谢中发现了广泛的物种间和物种内功能异质性.
- 发现了一种独特的Bifidobacterium longum类,可以代谢α-glucans.
- 在孟加拉国分离物中发现了独特的基因集群,用于糖和人乳寡糖体的利用.
结论:
- 这项研究提供了对Bifidobacterial碳水化合物代谢的全面基因组理解.
- 基于基因组学的基因糖利用预测通过体外实验得到了验证.
- 这些发现可以指导基于营养偏好的菌株特定益生菌和共生菌配方的合理设计.
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