鉴定参与 ingenane diterpenoid 生物合成的 BAHD-acyltransferase 酶
Carsten Schotte1, Matilde Florean1, Tomasz Czechowski2
1Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
The New phytologist
|July 17, 2025
概括
研究人员在Euphorbia peplus中确定了用于 ingenol-3-angelate生物合成的关键基因. 这一发现使得这种强大的抗瘤化合物的增强生产成为可能,并有助于发现相关的植物天然产品.
科学领域:
- 植物生物化学 植物生物化学
- 自然产品的生物合成.
- 分子生物学分子生物学
背景情况:
- 欧花科 (Euphorbiaceae) 家庭产生的复杂的二类植物具有重要的药用作用,包括抗癌性质.
- 从Euphorbia peplus中分离出来的 Ingenol-3-angelate 具有强大的抗瘤作用.
- 了解 ingenol-3-angelate 的生物合成对于其潜在的治疗应用至关重要.
研究的目的:
- 为了识别和描述参与Euphorbia peplus. ingenol-3-angelate生物合成的基因.
- 阐明负责形成 ingenol-3-angelate 和其衍生物的酶化步骤.
- 为代谢工程的 ingenol-3-angelate 生产奠定了基础.
主要方法:
- 在Nicotiana benthamiana的途径复制.
- 在体外测定与重组酶.
- 病毒诱导的基因沉默 (VIGS) 在E. peplus.
主要成果:
- 鉴定了两种催化基因的基因,从基因中形成 ingenol-3-angelate.
- 鉴定了三种涉及到 ingenol-3-angelate 到 ingenol-3-angelate-20-acetate 的乙化过程中的基因.
- 已经证明,EpBAHD-08对于天使化是必不可少的,而EpBAHD-06则影响了酸.
结论:
- 这项研究确定了英-3-酸盐生物合成的关键基因,包括必不可少的天使化酶EpBAHD-08.
- 对于乙化步骤存在功能冗余,如VIGS所示.
- 这项研究为增加ingenol-3-angelate水平和发现进一步的生物合成基因提供了基础.
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