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通过nh-PHIP检测血液中的腺酸

Helen Pais¹, Indrek Reile¹, Kerti Ausmees¹

¹National Institute of Chemical Physics and Biophysics, 12618 Tallinn, Estonia.

Analytical chemistry

|July 17, 2025

概括

非原性偏诱导极化 (nh-PHIP) 增强了用于血液分析的NMR光谱学. 这种方法成功地检测红细胞中的腺酸,为改善代谢学铺平了道路.

科学领域:

分析化学分析化学
生物化学生物化学
医学诊断医学诊断医学诊断

背景情况:

核磁共振 (NMR) 谱学提供了强大的分析能力,但往往受到低灵敏度的影响.
超极化技术显著提高了NMR信号,提高了灵敏度,分辨率,并缩短了采集时间.
超诱导极化 (PHIP) 是一种突出的超极化方法,其非化 (nh-PHIP) 变种提供了更广泛的适用性.

研究的目的:

应用和优化非化对诱导极化 (nh-PHIP) 技术用于血液样本的分析.
开发一种方法,在血液的红细胞 (RBC) 分数内敏感检测腺酸盐 (ATP,ADP,AMP).
证明nh-PHIP的可行性,用于分析血液中的红细胞和血成分的代谢学.

主要方法:

应用非化对诱导极化 (nh-PHIP) 的NMR光谱学.
开发样品制备协议,包括高效的红细胞溶解和甲醇-提取.
从ATP-Mg复合体中去除干扰Mg2+离子.
使用有机缓冲剂 (Et3N/Et3N-HCl) 来维持分析物的质子化状态.

主要成果:

在使用nh-PHIP的红细胞提取物中成功检测了腺三酸盐 (ATP),腺二酸盐 (ADP) 和腺单酸盐 (AMP).
建立了优化的样本准备协议,包括溶解和提取,与nh-PHIP兼容.
介绍了有史以来第一个nh-PHIP人体红细胞和人体血血小部分的NMR光谱.

结论:

nh-PHIP是一种可行的和强大的技术,用于增强血液成分的NMR分析.
开发的方法使得能够灵敏地检测血液中的关键代谢物,如腺酸.
这项工作代表了提高灵敏度,基于血液的NMR代谢学方面的重大进展.

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