相关实验视频

Updated: Sep 15, 2025

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

研究来自Helvella leucopus的多糖的化学修饰和修饰后的结构-活性关系

Wenwen Li¹, Hao Xie¹, Nuermaimaiti Yiliyasi¹

¹College of Biological Sciences and Technology, Yili Normal University, Yining, 835000 China.

|July 17, 2025

概括

从Helvella leucopus (HLP) 中提取多糖并进行化学修饰. 修改后的HLP显示显著增强了抗氧化剂和低血糖活性,这表明功能性食品和药物的潜力.

科学领域:

食品科学与技术食品科学与技术
生物化学生物化学
药理学药理学是指药理学的学科.

背景情况:

可食用和药用真菌的多糖具有宝贵的生物活性,包括抗氧化和降糖作用.
野生Helvella leucopus因其经济,营养和促进健康的潜力而闻名,引发了对其生物活性化合物的兴趣.

研究的目的:

为了从Helvella leucopus中提取和净化多糖 (HLP).
研究化学修饰对HLP的结构和生物活动的影响.
评估原生和改性HLP的抗氧化和低血糖潜力.

主要方法:

使用1-乙基-3-甲基化 (EMImBr) 和超声波进行HLP提取.
通过乙醇沉,Sevage脱蛋白化,透析和染色学 (DEAE-52,Sephadex G-100) 进行净化.
化学修改HLP,然后使用FT-IR,X射线衍射,SEM进行表征,并评估生物活动.

主要成果:

获得了均的HLP成分,化学修饰的衍生物显示出不同的单糖化合物组成和分子量.
在HLP及其衍生物之间,FT-IR和X射线衍射模式是相似的,而SEM显示了显著的表面形态变化.
无论是HLP还是其衍生品都表现出显著的抗氧化和降糖活性,改性版本显著增强了对α-葡萄糖酶和α-氨基酶的自由基清除和抑制作用.

结论:

化学修饰显著增强了来自Helvella leucopus的HLP的生物活动.
这些发现为了解多糖的结构-活性关系提供了理论基础.
高脂蛋白及其修饰衍生品显示出开发功能性食品和药物具有强大的抗氧化和降糖特性的前景.

关键词:

聚糖的化学修饰聚糖的化学修饰黑 (Helvella leucopus) 是一个白类动物.它是一种多糖化合物.结构性表征结构性表征

更多相关视频

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate CDAP

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate CDAP

Published on: June 14, 2021

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Hydrolysis

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...