相关实验视频

Updated: Sep 15, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

RtmR是一种嵌入膜的RRM家族RNA结合蛋白,调节生物膜形成

Jacob A Vander Griend^1,2, Hunter C Nottage¹, Andrew Mehle^1,2

¹Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI USA.

bioRxiv : the preprint server for biology

|July 17, 2025

概括

研究人员确定了RtmR,一种新的膜结合RNA结合蛋白,可以抑制海洋细菌Vibrio fischeri中的生物膜形成. 这种蛋白质通过控制RscS胺激酶来调节生物膜的发育.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌遗传学细菌遗传学

背景情况:

维布里奥·菲舍里 (Vibrio fischeri) 是一种模型生物体,用于研究细菌群体的行为,如定数感应和生物膜形成.
识别生物膜发育的负调节者对于理解细菌社区动态至关重要.

研究的目的:

为了识别Vibrio fischeri中的生物膜形成的新型负调节剂.
描述一种新发现的RNA结合蛋白的功能和机制,RtmR.

主要方法:

基因查以确定生物膜形成抑制剂.
蛋白质局部化研究和域分析 (RRM,RNP1,RNP2).
RNA结合试验和CLIP-seq用于识别RNA连接体.
分析RtmR对RscS活动和水平的影响.

主要成果:

一种与膜结合的RNA结合蛋白,RtmR,被确定为生物膜抑制剂.
RtmR的膜定位和RNA识别动机 (RNP1,RNP2) 对于其功能至关重要.
CLIP-seq确定了RscS mRNA作为一个RtmR目标.
RtmR通过负面调节RscS活动来抑制生物膜的形成.

结论:

RtmR是一种新型细菌RNA结合蛋白,可以控制生物膜的发育.
这项研究揭示了一条新的调节途径,涉及Vibrio fischeri生物膜形成中的RtmR和RscS.
这项工作扩大了对细菌群体行为中的RNA结合蛋白的理解.

关键词:

在RNA结合蛋白质中的RNA结合蛋白.维布里奥·菲舍里 (Vibrio fischeri) 是一种鱼类.细菌遗传学细菌遗传学生物膜是一种生物膜.信号传导的信号传导.这种共生是共生.

更多相关视频

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

相关概念视频

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...