在牛肉和猪肉加工厂的多种微生物群落的表征及其对病原体应激耐受性的影响
Rong Wang1, Sapna Chitlapilly Dass2, Vignesh Palanisamy2
1US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, United States.
Frontiers in microbiology
|July 17, 2025
概括
食品传播的病原体如大肠杆菌O157:H7和沙门氏菌可以在肉类植物中形成生物膜. 它们在消毒后的生存率各不相同,猪肉植物的恢复率高于牛肉植物,受到微生物群落的影响.
科学领域:
- 食品微生物学 食品微生物学
- 环境微生物学 环境微生物学
- 食品安全 食品安全
背景情况:
- 大肠杆菌O157:H7和沙门氏菌 (Salmonella enterica) 是重要的食物传播病原体.
- 肉类加工设施中的生物膜形成可能导致产品受到污染.
- 病原体的应激耐受性受到多种微生物群落和加工条件的影响.
研究的目的:
- 描述肉植物排水中的微生物,并评估它们对病原体消毒剂耐受性的影响.
- 在加工条件下,研究大肠杆菌O157:H7和沙门氏菌在多种群体中的附着和生存.
- 评估多组分消毒剂在牛肉和猪肉加工环境中对附着病原体的有效性.
主要方法:
- 从牛肉和猪肉加工厂的地板下水道收集自然微生物.
- 在低温下,病原体在接触表面的附着和生存的表征.
- 多组分消毒剂的应用和病原体减少和恢复的评估.
- 扫描电子显微镜 (SEM) 和微生物群落的元基因组分析.
主要成果:
- 病原体有效地集成到表面的多种群体中,沙门氏菌的细胞密度高于大肠杆菌O157:H7.7.
- 消毒剂有效地减少了粘附的病原体,但在猪肉植物样本中,与牛肉植物样本相比,观察到更高的存活率和恢复率.
- 表面地形影响了微殖民地形态和细菌耐受性.
- 伪杆菌家族,杆菌家族和肠杆菌家族是最丰富的细菌家族;物种丰富度各不相同.
结论:
- 多种微生物群落和物种间的相互作用显著影响肉类加工厂对消毒剂的病原体耐受性.
- 对于不同的加工设施,应逐个评估卫生效率和病原体流行预防策略.
- 了解微生物群落动态对于在肉类生产环境中有效控制食源性病原体至关重要.
相关概念视频
Other Stress Responses in Bacteria
70
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
70
Microorganisms in Agriculture and Food industry
401
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
401


