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Updated: Sep 15, 2025

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

基于细胞的ELISpot协议,用于检测和量化小鼠全器官单细胞悬浮体中的抗原特异性抗体分泌细胞

Christina Brunner¹, Shannon R Menzel¹, Franziska Rost¹

¹Division of Molecular Immunology, Department of Medicine 3 - Rheumatology and Immunology, Universitätsklinikum Erlangen, Nikolaus-Fiebiger Center, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany.

|July 17, 2025

概括

这项研究详细介绍了一种使用酶相关免疫位点 (ELISpot) 测定方法量化抗体分泌细胞 (ASC) 的协议. 这种方法对于理解疫苗反应和疾病研究中的适应性免疫是至关重要的.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

抗体分泌细胞 (ASC) 是适应性免疫反应的关键组成部分.
了解ASC对于评估疫苗疗效和研究免疫相关疾病至关重要.

研究的目的:

提出一个标准化的协议来量化ASCs.
为了能够准确地列出ASC,用于研究应用.

主要方法:

酶相关免疫位点 (ELISpot) 试验的详细方案.
包括细胞样本准备和ELISpot板量化的步骤.

主要成果:

ELISpot测定提供了一种可靠的方法来量化ASCs.
该协议适用于各种研究环境.

结论:

提出的ELISpot协议为免疫学研究提供了有价值的工具.
促进对适应性免疫,疫苗反应和ASC相关病理学的研究.

关键词:

抗体是对抗体的一种.免疫学免疫学免疫学

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Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation

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