延迟胚胎附着,循环与怀孕相关的葡萄糖蛋白,以及母牛胚胎接受者的怀孕流产
João Paulo N Andrade1, Rafael R Domingues2, Victor E Gomez-León3
1Department of Animal and Dairy Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI, 53706, USA; Department of Agricultural Sciences, West Texas A&M University, Canyon, TX, 79016, USA.
Theriogenology
|July 17, 2025
概括
胚胎接受者的妊娠损失与较低的妊娠相关糖蛋白 (PAG) 有关,这表明胎盘不理想. 早期孕激素 (P4) 补充改善了每胚胎移植 (P/ET) 的怀孕,这些母牛的PAG水平较低.
科学领域:
- 生殖生物学 生殖生物学
- 胚胎转移技术的使用
- 牛的繁殖 牛的繁殖
背景情况:
- 失去怀孕 (PL) 是胚胎移植计划的一个重要问题.
- 怀孕相关的葡萄糖蛋白 (PAG) 是胎盘功能的关键指标.
- 孕激素 (P4) 补充剂通常用于支持早期怀孕.
研究的目的:
- 在胚胎接受者中研究PAG和PL之间的关系.
- 评估早期P4补充剂对每次胚胎移植 (P/ET) 和PL对怀孕的影响.
主要方法:
- 实验1:回顾性队列研究1003个新鲜的试管婴儿胚胎转移在荷尔斯坦公牛,分析PAGs和PL.
- 实验2:对540头公牛进行随机对照试验,比较14-19天的P4补充剂 (PlusP4) 与对照.
主要成果:
- 在转移后21-28天,较低的PAG水平与PL (28-33d和33-47d) 的增加有关.
- 早期的P4补充 (14-19d) 在28d显著增加了胚胎附着 (Eatt) 和P/ET.
- 补充P4改善了所有PAG三角体中的P/ET,在PAG水平较低的小牛中最显著的好处.
结论:
- 低PAG表示的低最佳胎盘,是胚胎接受者早期怀孕损失的关键因素.
- 早期的P4补充是改善P/ET的有效策略,特别是在表现出胎盘发育不佳的雌牛中.
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