忽视了肠道细菌作为水生环境中抗菌素耐药性的宿主
Anca Farkas1,2, Anca Butiuc-Keul3,4, Rahela Carpa3,4
1Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 1 M. Kogălniceanu Street, 400084, Cluj-Napoca, Romania. ancuta.farkas@ubbcluj.ro.
Scientific reports
|July 17, 2025
概括
环境细菌,包括被忽视的物种,表现出显著的抗生素耐药性 (AMR) 和多药耐药性 (MDR). 遗传多样性分析揭示了基因组可塑性,抗性降低和生物膜形成之间的相关性.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 (AMR) 研究研究
- 细菌遗传学和基因组学 细菌遗传学和基因组学
背景情况:
- 抗生素耐药细菌 (ARB) 和它们在环境微生物群中的传播对健康构成重大风险.
- 目前的水质和AMR监测主要关注肠杆菌和大肠杆菌,忽视其他物种.
- 环境隔离物可以含有多种抗生素耐药性基因 (ARG),并表现出不同的耐药性概况.
研究的目的:
- 研究被忽视的环境细菌物种中的抗生素耐药性和生物膜形成.
- 评估这些分离物的遗传多样性和分子类型.
- 探索遗传多样性,抗生素耐药性和生物膜形成之间的相关性.
主要方法:
- 从六种被忽视的物种中分离和识别了14种环境细菌菌株.
- 抗生素耐药性分析 (表型和基因型) 和ARG的检测.
- 肠杆菌重复性跨基因共识 (ERIC) -PCR用于遗传多样性评估和分子类型.
- 生物膜形成试验.生物膜形成试验.
主要成果:
- 确定的物种包括克罗诺巴克特·萨卡扎基 (Cronobacter sakazakii),克鲁伊维拉中间体 (Kluyvera intermedia),莱克莱西亚·阿德卡尔博克西拉塔 (Leclercia adecarboxylata),罗乌特拉·奥尔尼丁诺利蒂卡 (Raoultella ornithinolytica),罗乌特拉·特里根纳 (Raoultella terrigena) 和耶尔西尼亚·马西利尼斯 (Yersinia massiliensis) 等.
- 所有分离物都表现出对红红素的内在耐药性和对安培素和四环素的耐药性增加;没有一种耐卡巴.
- 在14个分离物中,有10个是多药耐药 (MDR),24个ARG中有13个被检测到,包括blaTEM,blaCTX-M,tet,sul,erm, mef和qnr基因.
- 现型和基因型耐药性之间存在强烈的正相关性.
- ERIC-PCR有效地分化了分离物,显示了ERIC带数,抗性大小和生物膜得分之间的负相关性,这表明更高的基因组可塑性与更低的抗性和粘附相关.
结论:
- 被忽视的环境细菌物种具有显著的AMR和MDR特征.
- 通过ERIC-PCR评估的遗传多样性与耐药性和生物膜形成有关.
- 高基因组可塑性可能会赋予适应性,但可能会限制特定的生存策略,如高耐药性或强大的生物膜形成.
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