使用提取和离子选择性电极进行植物材料化物分析的简单和低成本方法
Chenyu Zhang1,2, Mark G M Aarts1, Antony van der Ent3
1Laboratory of Genetics, Wageningen University and Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands.
Plant methods
|July 17, 2025
概括
一种新的,具有成本效益的方法简化了植物中的化物确定. 该技术使用氧化提取和离子选择性电极,为植物化物分析提供可靠的替代方案.
科学领域:
- 分析化学 分析化学
- 植物科学 植物科学
- 环境科学 环境科学
背景情况:
- 植物中传统的化物测定方法昂贵而复杂.
- 需要可访问和负担得起的分析技术来检测植物化物含量.
研究的目的:
- 开发一种简单,具有成本效益和可靠的方法,用于植物样本中的化物 (F−) 分析.
- 为了优化提取和测量参数,以准确量化化物.
主要方法:
- 优化了氧化提取 (5mol·L-1) 与加热 (120°C 0.5小时).
- 使用酸来调整离子强度.
- 使用离子选择性电极进行化物测量.
- 使用经过认证的参考材料和直角测试设计验证了该方法.
主要成果:
- 达到了1.41 mg·kg-1的检测极限 (LOD) 和4.71 mg·kg-1.1的量化极限 (LOQ).
- 在 *Arabidopsis thaliana* 和 *Camellia sinensis* 中证明了高恢复率 (83.5391.55%),相对标准偏差较低 (RSD ≤4.98%).
- 在*A. thaliana* (16.00 mg·kg−1) 和*C. sinensis* (292.71 mg·kg−1) 中的量化含量,在化处理的溶液中生长,以及在商业茶叶产品中生长 (16.2861.78 mg kg−1).
结论:
- 开发的方法对于植物化物分析来说简单,可靠,且具有成本效益.
- 这种方法为昂贵和复杂的现有方法提供了切实可行的替代方案.
- 建议进一步进行实验室间验证,以使其成为标准化方法.
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