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相关概念视频

Subviral Agents01:29

Subviral Agents

115
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
115

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相关实验视频

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Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
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病毒镜TM:使用HTS和基于云的分析,为植物病毒和病毒病毒诊断提供通用解决方案.

Verónica Morgante1, Juan Cristóbal Jiménez1, Claudio Ponce1

  • 1Multiplex SpA, Santiago, Chile.

Frontiers in microbiology
|July 18, 2025
PubMed
概括

病毒镜TM使用基于云的高通量测序来准确检测植物病毒和病毒,提高生物安全性. 这种先进的诊断工具增强了新出现的病毒的检测,保护了食品供应链.

关键词:
RT-qPCRR 是一个人.病毒扫描仪 病毒扫描仪边境安全 边境安全基因组组装的覆盖范围高通量测序 (HTS) 是一种高通量测序.植物检疫检疫 植物检疫检疫植物健康问题 植物健康复制案例识别 复制案例识别

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科学领域:

  • 植物病理学 植物病理学
  • 基因组学就是基因组学.
  • 生物安全是生物安全.

背景情况:

  • 国际植物贸易增加了植物病毒传播的全球风险,威胁到粮食安全.
  • 高通量测序 (HTS) 提供先进的病毒诊断,但面临数据分析和实施障碍.
  • 病毒镜TM是一项云服务,旨在克服HTS的局限性,以可靠地识别病毒和病毒.

研究的目的:

  • 评估ViroscopeTM算法用于植物病毒和植物病毒在进入后隔离环境中的植物卫生诊断.
  • 评估ViroscopeTM在检测受调和新兴病毒病原体方面的性能.
  • 将ViroscopeTM的诊断能力与RT-qPCR等传统方法进行比较.

主要方法:

  • 使用Illumina®平台对来自入境后隔离的144个植物组织样本进行深度测序.
  • 使用ViroscopeTM算法和传统RT-qPCR方法并行分析样本.
  • 评估ViroscopeTM的检测能力,包括低病毒丰度和新兴病毒.

主要成果:

  • 28.5%的被隔离植物携带了受管制和/或新出现的病毒和病毒病毒.
  • 病毒镜TM在25%的植物中检测到新兴病毒,突显了传统方法错过的风险.
  • 病毒镜TM与RT-qPCR有很高的相关性,并通过RT-qPCR检测出样本中呈阴性病毒.

结论:

  • 病毒镜TM提高了病毒和病毒病毒检测的准确性,并为植物卫生诊断提供了宝贵的生物学见解.
  • 基于云计算的平台通过使诊断变得用户友好和高效,促进全球采用HTS技术.
  • 病毒镜TM通过识别传统方法可能忽略的潜在风险,包括新出现的病毒,来提高生物安全.