通过miR-135b-5p/FRK轴,CircYAP1可以抑制骨肉瘤的进展
Mingrui Du1,2, Xuesan Zhang1, Menglin Cong3
1Department of Orthopedics, The 960th Hospital of the PLA Joint Logistics Support Force, Ji'nan 250000, Shandong, China.
iScience
|July 18, 2025
概括
循环RNA YAP1 (circYAP1) 通过抑制细胞增殖和转移来抑制骨髓瘤的进展. 这项研究揭示了circYAP1作为骨髓瘤治疗的潜在治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 在骨髓瘤中circYAP1的特定功能在很大程度上仍未被探索.
研究的目的:
- 研究circYAP1在骨髓瘤中的作用和机制.
- 探索circYAP1作为骨髓瘤的潜在治疗点.
主要方法:
- 定量实时PCR用于评估骨髓瘤组织和细胞系中的circYAP1表达.
- 在体外测试 (扩散,入侵,迁移) 来评估circYAP1的功能.
- 机制研究涉及RNA免疫沉降和光酶记者测试.
- 在小鼠的正位体肺转移模型.
主要成果:
- 在骨髓瘤组织中,circYAP1表达显著下调,特别是在转移和晚期样本中.
- 过度表达circYAP1抑制了骨髓瘤细胞的增殖,入侵和迁移.
- circYAP1通过菌miR-135b-5p作为竞争的内源RNA (ceRNA) 起作用,导致其基因FRK.的调节.
- circYAP1在正位肺转移模型中表现出瘤抑制作用.
结论:
- 在骨髓瘤中,circYAP1充当瘤抑制剂.
- 环YAP1/miR-135b-5p/FRK轴在骨髓瘤进展中起着至关重要的作用.
- circYAP1代表了骨髓瘤的一个有前途的治疗点.
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