基于Mg2+介导的CRISPR/Cas12a和Au@Pt纳米颗粒的三模生物感知策略用于乙型肝炎病毒
Chenggong Zhu1,2, Qunfang Huang1,2, Ruijie Fu3,4
1Department of Laboratory Medicine, Gene Diagnostic Research Center, Fujian Key Laboratory of Laboratory Medicine, Fujian Clinical Research Center for Laboratory Medicine of Immunology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China.
Small (Weinheim an der Bergstrasse, Germany)
|July 18, 2025
概括
一个新的生物传感策略结合了CRISPR/Cas12a,循环介导的同热放大 (LAMP) 和纳米粒子,用于快速地在现场检测乙型肝炎病毒 (HBV) 的DNA. 这种方法提供了高灵敏度和准确性,以改善公共卫生监测.
科学领域:
- 生物技术是生物技术.
- 分子诊断学 分子诊断学
- 纳米技术纳米技术
背景情况:
- 乙型肝炎病毒 (HBV) 感染是一个主要的全球健康问题,需要快速检测DNA以控制.
- 传统的HBV DNA检测方法缺乏现场诊断所需的便携性和速度.
研究的目的:
- 开发一种新的,高度敏感的,便携式的生物传感平台,用于乙型肝炎病毒DNA检测.
- 整合集群定期间隔的短Palindromic重复/关联蛋白12a (CRISPR/Cas12a) 与循环介导同热放大 (LAMP) 和纳米粒子报告器.
主要方法:
- 使用Mg2+增强的CRISPR/Cas12a跨裂变活性.
- 结合CRISPR/Cas12a与LAMP和Au@Pt纳米粒子进行信号放大和报告.
- 启用多个信号读取:紫外线光谱,RGB颜色和温度.
主要成果:
- 在三个检测模式中,HBV DNA的线性检测范围从50到10,000副本/μL不等.
- 证明的低检测极限: 24.07 副本/μL (UV-vis), 39.65 副本/μL (RGB) 和 23.33 副本/μL (光热).
- 在48个具有高灵敏度 (高达100%) 的血清样本中验证了生物传感策略,用于定性HBV DNA检测.
结论:
- 开发的三模生物传感策略为快速,便携和敏感的核酸检测提供了一个有前途的工具.
- 这一下一代LAMP-CRISPR/Cas12a平台在现场诊断和公共卫生应用方面具有重大潜力.
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