莉花的信号与SOD7-KLU通路坐标,以调节Arabidopsis thaliana中的种子大小
Juping Zhang1,2, Jian Yao3, Kunrong He1,4
1CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
The Plant cell
|July 18, 2025
概括
通过CORONATINE INSENSITIVE1 (COI1) 介导的雅斯蒙酸信号,通过抑制KLUH (KLU) 表达,在母体上抑制了Arabidopsis种子的生长. 这一途径涉及JASMONATE ZIM-DOMAIN (JAZ) 抑制剂和MYC转录因子,与SOD7/DPA4协调调节种子发育.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 种子大小是作物产量和植物适应性的关键决定因素.
- 众所周知,植物激素斯莫纳酸调节了Arabidopsis thaliana的种子大小,但其分子机制尚未完全理解.
研究的目的:
- 阐明斯酸盐信号调节阿拉比多普西斯莉亚种子大小的分子机制.
- 为了确定关键的基因和蛋白质相互作用参与这个调节途径.
主要方法:
- 研究了CORONATINE INSENSITIVE1 (COI1) 介导的莉花酸信号在母亲抑制种子生长中的作用.
- 分析了JASMONATE ZIM-DOMAIN (JAZ) 抑制剂,DA1-1 (SOD7) 的转录因子抑制剂和与APEX4 (DPA4) 和KLUH (KLU) 发展相关的PCG目标之间的相互作用.
- 研究了SOD7与MYC2和MYC4的关联,这些关键的莉花信号调节器,以及它们对KLU转录和种子生长的影响.
主要成果:
- 通过COI1传递的斯蒙酸信号,通过抑制KLU表达来抑制母体种子生长.
- SOD7和DPA4直接抑制KLU,而SOD7的过度表达挽救了Coi1突变的种子大小缺陷.
- SOD7和MYC2/MYC4协同降低KLU转录并抑制种子生长,而JAZ1干扰了这一过程.
- 在盐度压力下,雅斯蒙酸信号影响种子大小,由SOD7和KLU介导.
结论:
- 通过COI1受体发送的斯蒙酸信号,通过调节KLUH表达来控制母体的种子大小.
- 贾斯抑制剂和MYC转录因子与SOD7/DPA4-KLU通路相互作用,将贾斯酸信号整合到种子发育中.
- 这一途径对于种子大小调节至关重要,特别是在盐度等环境压力下.
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