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Updated: May 5, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

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选择性蛋白酶基质的鉴定

Franco F Faucher1,2, Kristýna Blažková2, Scott Lovell2,3

  • 1Department of Chemistry, Stanford University, Stanford, California 94305, United States.

Journal of the American Chemical Society
|July 18, 2025
PubMed
概括

这项研究引入了一种新的菌体显示方法,使用化学修饰的循环来发现高度选择性的蛋白酶基质. 这种方法克服了传统方法的局限性,为生物学和医学提供了新的工具.

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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