产前压力会增加子女的皮质水平,因为它会损害胎盘的葡萄皮质皮质屏障功能
Can Liu1,2, Hongya Liu3,1, Hongyu Li1,2
1School of Public Health, Ningxia Medical University, Yinchuan, Ningxia, China.
PloS one
|July 18, 2025
概括
在大鼠中产前压力 (PS) 改变了胎盘基因甲基化,增加了后代的皮质水平. 这种干扰会影响葡萄糖皮质体屏障,影响发育.
科学领域:
- 生殖生物学 生殖生物学
- 发育神经科学的发展神经科学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 产前压力 (PS) 与不良后代结果有关.
- 胎盘葡萄糖皮质体 (GC) 屏障调节胎儿暴露于母亲的GCs.
- 表观遗传修饰,如DNA甲基化,可能会调解PS效应.
研究的目的:
- 为了研究PS和皮质水平之间的关联.
- 检查PS对胎盘GC屏障基因DNA甲基化的影响.
- 分析关键的胎盘GC相关基因的基因和蛋白质表达.
主要方法:
- 对于PS而言,慢性不可预测的轻度压力 (CUMS) 的老鼠模型.
- 减少表示双硫酸盐测序 (RRBS) 和MethylTargetTM测序用于DNA甲基化分析.
- 定量实时PCR (qRT-PCR) 和西式涂抹用于基因和蛋白质表达分析.
主要成果:
- 在母体和后代中PS血皮质的升高.
- 压力较大的母体的后代表现出改变的DNA甲基转移酶表达 (DNMT1上升,DNMT3A/3B下降).
- 在受压力的后代中,ABCB1和FKBP5基因被超甲基化,已确认有差异甲基化位点.
结论:
- 通过由高甲基化驱动的异常基因表达,PS破坏了胎盘GC屏障.
- 由于胎盘屏障的破坏,后代的皮质水平升高可能会影响生长和发育.
- 这项研究提供了关于表观遗传机制的证据,该机制将产前压力与改变后代GC暴露联系起来.
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