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通过准TNF-α/NF-κB/CCL5信号轴,大麻 (CBD) 对骨肉瘤的抗瘤作用

Fan Yang¹, Shuqin Duan², Jingwei Liu³

¹State Key Laboratory of Frigid Zone Cardiovascular Diseases (SKLFZCD), Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Heilongjiang, 150081, China; Northern Translational Medicine Research and Cooperation Center, Heilongjiang Academy of Medical Sciences, Harbin Medical University, Harbin 150081, China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|July 18, 2025

概括

大麻素 (CBD) 通过向TNF-α/NF-κB/CCL5通路,有效地抑制骨髓瘤的生长. 这项研究揭示了CBD破坏炎症信号,为骨髓瘤治疗提供了一个有前途的新疗法途径.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

骨髓瘤由于其侵略性和高转移潜力而构成重大治疗挑战.
新的治疗策略对于改善骨髓瘤患者的治疗结果至关重要.
大麻素 (CBD) 是一种非精神活性大麻素,具有临床前的抗癌活性,但其在骨髓瘤中的机制需要阐明.

研究的目的:

为了研究大麻素 (CBD) 对骨髓瘤的抗瘤作用.
在骨髓瘤中识别TNF-α/NF-κB/CCL5信号轴内CBD的分子标.
阐明CBD在骨髓瘤治疗中的治疗潜力.

主要方法:

细胞增殖,迁移和入侵试验被用来评估CBD对骨髓瘤恶性瘤的影响.
采用老鼠异种移植模型来评估CBD的体内疗效.
网络药理学,RNA-seq,ELISA,qRT-PCR,西部斑块,CETSA,SPR,ITC和分子对接被用来识别和验证分子点,特别是p65 (NF-κB子单元).

主要成果:

CBD显著抑制了骨髓瘤细胞的增殖,迁移和入侵,并在体内抑制了瘤的生长.
通过直接与p65结合,CBD破坏了TNF-α/NF-κB/CCL5轴,从而减少了NF-κB介导的CCL5转录.
在p65和CCL5之间的一个新的正反循环,对于骨髓瘤的炎症信号至关重要,被CBD废除.

结论:

这项研究表明,CBD通过向TNF-α/NF-κB/CCL5轴来抑制骨髓瘤的进展.
CBD破坏了一个协调的炎症-扩散级联,提供了一个新的治疗机制.
CBD成为骨髓瘤的有希望的治疗候选者,值得进一步临床研究.

关键词:

CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 大麻二醇 (Cannabidiol) 是一种在 NF-κBB 中.骨质肉瘤骨质肉瘤是什么瘤转移的发生.

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