败血症和败血性休克的诊断生物标志物:基于NMR的血清代谢学研究
Swarnima Pandey1, Afzal Azim2, Neeraj Sinha1
1Centre of Biomedical Research, SGPGIMS Campus, Raebareli Road, Lucknow 226014, India.
Biophysical chemistry
|July 18, 2025
概括
核磁共振 (NMR) 确定了败血症和败血性休克的血清生物标志物. 酸盐/乳酸盐和氨/氨酸的比率有效地区分疾病的严重程度,并与患者的结果相关联.
科学领域:
- 生物化学 生物化学
- 医学诊断 医学诊断 医学诊断
- 密集护理医学 密集护理医学
背景情况:
- 败血症和败血性休克是重症监护室死亡的主要原因.
- 氧化应激显著导致败血症的发病,需要新的诊断方法.
- 目前对败血症和败血症休克的治疗方法存在局限性,这凸显了需要改进生物标志物的需要.
研究的目的:
- 调查基于核磁共振 (NMR) 的血清生物标志物用于败血症和败血性休克.
- 通过代谢分析,探索氧化应激在败血症发病过程中的作用.
- 评估特定代谢物比率在败血症和败血症休克中的诊断和预后潜力.
主要方法:
- 来自41名败血症,21名败血症和16名疾病控制患者的血清样本使用1D H Carr Purcell Meiboom Gill (CPMG) NMR光谱学进行了分析.
- 对代谢物的定量评估,比较了酸盐/乳酸盐 (Pyr/Lac) 和氨酸/氨酸 (Phe/Tyr) 的比例.
- 接收器操作特征 (AUROC) 分析评估了这些比率的歧视潜力.
主要成果:
- 在败血症休克中,Pyr/Lac比率最低,其次是败血症和对照.
- 在败血症休克中,Phe/Tyr比率最高,其次是败血症和对照.
- 这两种比率都显示出高的区分潜力,并与APACHE II分数相关,表明乳酸脱酶升高和氨酸氧酶活性降低.
结论:
- 基于NMR的血清代谢物比率 (Pyr/Lac和Phe/Tyr) 作为败血症和败血性休克的有效生物标志物.
- 这些生物标志物可以帮助诊断,预后和监测疾病活动和治疗反应.
- 这些发现表明氧化应激在败血症病理生理学中起着重要作用,反映在酶活动的改变中.
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