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一种新型抗微生物NDYT-8具有来自Bacillus subtilis natto的广谱活性:抗菌机制和序列简化

Yunxin Yi¹, Dongning Liu¹, Chao Cheng¹

¹School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

International journal of food microbiology

|July 18, 2025

概括

从Bacillus subtilis natto中分离出来的一种新型抗微生物,NDYT-8,显示出对食源性病原体的广泛活性. 这种有效地通过增加膜透性来保存食物,并显示出作为天然食品防腐剂的潜力.

科学领域:

微生物学微生物学
生物化学生物化学
食品科学食品科学食品科学

背景情况:

抗生素耐药性是一个日益增长的全球健康威胁.
抗微生物 (AMP) 为传统抗生素提供了一个有希望的替代品.
细菌素 natto是生物活性化合物的来源,具有潜在的应用.

研究的目的:

从Bacillus subtilis natto.中分离和描述一种新的抗微生物.
评估已识别的的抗菌活性和作用机制.
为了评估作为食品防腐剂的有效性.

主要方法:

抗微生物NDYT-8的分离和鉴定.
全基因组测序和分析.
确定针对食物传播病原体的最小抑制度 (MIC).
涉及膜透性测试的机制研究.
在豆腐保存模型中的应用.
结构与活动关系分析.

主要成果:

NDYT-8对大肠杆菌和沙门氏菌有广泛的抗菌活性 (MIC: 16μg/mL).
机制涉及增加的膜透性,导致细胞内容的泄漏.
在豆腐模型中,20 mg/mL的NDYT-8在2小时内减少了87.0%的细菌数量,有效性持续了10小时.
阳性电荷/性氨基酸对抗菌疗效至关重要;较短的变体 (RKILLMVK) 保持了活性.

结论:

NDYT-8是一种新型,高效的抗微生物,具有广泛的活性.
该通过破坏细菌膜完整性来起作用.
作为食品的天然防腐剂,NDYT-8具有显著的潜力.
结构-活性关系研究为设计改进的抗微生物提供了洞察力.

关键词:

抗菌是一种抗菌.细菌细菌纳托 natto.细胞膜的细胞膜.基因组基因组基因组基因组基因组结构与活动的关系.

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