对梨子中由Debaryomyces hansenii诱导的疾病耐药性的生理机制和转录组分析
Lina Zhao1, Xiaoyue Ren1, Ruyu Wen1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, People's Republic of China.
International journal of food microbiology
|July 18, 2025
概括
德巴里奥梅西斯汉森尼通过增强防御酶和激活信号通路来增强梨病的抵抗力. 这种酵母提供了一种安全的生物控制策略来对抗收获后的病原体.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 收获后的疾病对水果的质量和保质期产生重大影响.
- 德巴里奥梅西斯汉塞尼是一种已知的收获后病原体的对手.
- 梨中的D. hansenii诱导的耐药性的机制尚未完全理解.
研究的目的:
- 调查果中D. hansenii诱导的疾病耐药性的生理和分子机制.
- 为了阐明D. hansenii是如何触发针对Penicillium expansum等病原体的防御反应的.
主要方法:
- 抑制对抗Penicillium expansum. 的测定.
- 对酶活性和代谢物含量的生理分析.
- 转录组分析以识别激活的信号通路和基因表达.
主要成果:
- D. hansenii抑制了P. expansum,殖民了水果表面,并争夺了资源.
- 与耐药性相关的酶 (PPO,PAL,POD) 的增强活性和二次代谢物 (,黄) 的增加.
- 上调的抗氧化酶 (SOD,CAT,APX) 清除活性氧物种 (ROS) 和激活的防御通路,包括素酸 (JA) 信号传递.
结论:
- D. hansenii诱导梨对收获后病原体的多维抗性.
- 酵母激活了酶和分子防御机制,包括JA信号.
- 为开发使用D. hansenii用于梨保存的生物控制策略提供了基础.
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