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物理,转录和MdABR1-介导的机制是"富士"果中抗性的基础

Wanying Xie¹, Jie Shen¹, Yu Tian¹

¹School of Horticulture, Ludong University, 186 Hongqizhong Road, Yantai, Shandong Province 264025, China.

Plant science : an international journal of experimental plant biology

|July 18, 2025

概括

的毒性严重影响果的生长,损害光合作用,导致营养不平衡. 果树激活抗氧化防御和特定的基因,如MdABR1,以管理压力和适应.

科学领域:

植物科学植物科学
环境科学环境科学
分子生物学分子生物学

背景情况:

(Mn) 毒性是一个重要的农业问题,降低了果的产量和质量.
果树具有对的吸收和内部平衡的调节机制,但它们的应激反应尚未完全理解.
关于"富士"果树对Mn压力的生理和转录基因适应的研究有限.

研究的目的:

为了研究"富士"果树在压力下的生理和转录基因反应.
确定关键的基因和分子途径,参与Mn排毒和应激适应.
为果对Mn毒性的反应构建一个系统性调节模型.

主要方法:

有控制的实验使果苗暴露在Mn压力下.
生理学测量包括生长,积,H2O2水平,营养含量和光合作用色素分析.
转录组分析 (RNA-Seq) 来识别差异表达基因 (DEG) 和通路丰富 (GO,KEGG).
使用酵母基因转化和烟草中短暂表达的候选基因 (例如,MdABR1) 的功能验证.

主要成果:

的压力抑制了果苗的生长,增加了和H2O2的积累,导致营养不平衡,并减少了光合作用.
抗氧化酶的升级和抗氧化剂的积累有助于减轻Mn诱导的氧化应激.
确定了458个DEG,包括AP2/ERF家族基因和金属结合蛋白,在细胞壁生物合成,烯酸生物合成和脂肪酸延长中具有丰富的途径.
证实MdABR1可以减少的生物积累和毒性.

结论:

果树对的毒性表现出复杂的生理和分子反应,包括抗氧化防御和特定的基因调节.
该研究阐明了关键的分子机制,包括MdABR1在Mn排毒和适应中的作用.
开发了一个系统性监管模型,为培育耐米果品种提供了基础.

关键词:

马卢斯家中的家禽.的毒性和耐受性生理反应反应的生理反应对转录组进行分析.

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