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改进了工程菌体编码的内素的性作用,并对其对细菌糖的作用进行了对接

Ruma Rani¹, Vikram Poria¹, Anu Bala Jaglan¹

¹National Centre for Veterinary Type Cultures (NCVTC), ICAR-National Research Centre on Equines, Hisar 125001, India.

International journal of biological macromolecules

|July 18, 2025

概括

工程内素显示出对抗抗生素耐药的沙门氏菌有前途. 一种经过修改的与多性非 (PCNP) 结合的内溶酶表现出增强的抗菌活性和稳定地与细菌细胞壁组件结合.

科学领域:

微生物学微生物学
生物技术是生物技术.
分子生物学分子生物学

背景情况:

沙门氏菌的抗菌素耐药性对全球健康构成重大威胁.
传统的菌体疗法面临着挑战,需要新的抗微生物策略.
来自菌体的工程内素提供了对抗耐药细菌的强有力的替代方案.

研究的目的:

设计,开发和评估一种由细菌衍生而来的,用多性非 (PCNP) 修改的工程内素.
为了增强内素对沙门氏菌的抗菌和性活性.
用计算方法研究改性内素的结合亲和力和结构稳定性.

主要方法:

结核素 φSE218_Lys76及其修改版 φSE218_Lys76_L1_PCNP 的克隆,表达和净化.
在体外评估活体和热杀死沙门氏菌细胞的抗菌和菌活性,有或没有EDTA治疗.
分子对接和动力学模拟以分析与酸甘碎片的结合亲和力.

主要成果:

与原始内素相比,修改后的内素 φSE218_Lys76_L1_PCNP 呈现出明显增强的溶解活性.
增强的活性归因于PCNP修饰和负电荷细菌表面之间有利的静电相互作用.
计算研究显示,改性内素与糖构建块 (NAG-NAM四聚合物) 的高结合亲和力 (-7.19 kcal/mol) 和结构稳定性.

结论:

聚化非的修改有效地增强了细菌衍生型内素的抗菌功效.
工程内素是一种有前途的替代抗微生物策略,用于对抗沙门氏菌等抗生素耐药性病原体.
该研究验证了将蛋白质工程与计算建模相结合的潜力,用于开发新型抗菌剂.

关键词:

抗微生物耐药性抗微生物耐药性细菌菌体是一种细菌.人工合成的内素.分子对接是分子对接.分子动态模拟分子动态模拟类甘油可以聚化非的多化非

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